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. 2021 Aug 12;9:681790. doi: 10.3389/fcell.2021.681790

FIGURE 4.

FIGURE 4

M5Ab inhibits Ang II-induced expression of pyroptosis-related inflammatory factors in mice by blocking the expression of OPN-N. (A,B) Immunohistochemistry staining of NLRP3, Caspase-1, ASC, IL-1β, and IL-18 in mice under different treatments, quantification of these pyroptosis-related inflammatory factors area (below). Scar bar 25 μm. (C,D) Western blotting analyses of the protein levels of NLRP3, ASC, IL-1β, IL-18, pro-Caspase-1, and GSDMD in mice, and quantification of these pyroptosis-related inflammatory proteins expression. β-actin was used as an internal control. Data are expressed as means ± SEM; n = 3 per group. ∗P < 0.05 vs the Ang II + M5Ab group; ∗∗P < 0.05 vs the control and control + M5Ab groups. Abbreviations: Ang II, angiotensin II; IL, interleukin; NLRP3, NOD-, LRR-, and pyrin domain-containing protein 3; and L, lumen.