Skip to main content
. 2021 Aug 12;9:681790. doi: 10.3389/fcell.2021.681790

FIGURE 5.

FIGURE 5

M5Ab attenuates proinflammatory cytokine gene expression and inflammatory cell infiltration by blocking the expression of OPN-N. (A) qPCR analyses of the mRNA expression levels of TNF-α, IL-6, and MCP-1 in mice under different treatments. (B,C) Immunohistochemistry staining of F4/80 macrophages in mice under different treatments, quantification of these F4/80 area. Scar bars: 200 μm (boxed areas), 25 μm (main images). (D) Immunohistochemistry staining and (E) Immunofluorescence staining of α9β1 in mice under different treatments. Scar bars: 200 μm (above), 50 μm (below). DAPI staining of nuclei:blue; α9β1 staining:red. Data are expressed as means ± SEM; n = 3 per group. ∗P < 0.05 vs the Ang II + M5Ab group; ∗∗P < 0.05 vs the control and control + M5Ab groups. Abbreviations: Ang II, angiotensin II; IL, interleukin; MCP, monocyte chemoattractant protein; TNF, tumor necrosis factor; L, lumen.