FIGURE 6.

M5Ab inhibits MMP2 and MMP9 expression in mice by blocking the expression of OPN-N. (A,B) Immunohistochemistry staining of MMP2 and MMP9 in mice under different treatments (A), quantification of MMP2 and MMP9 area (B). Scar bar 100 μm. (C) Western blotting analyses the protein levels of MMP2 and MMP9 in mice, and quantification of MMP2 and MMP9 protein expression. β-actin was used as an internal control. Data are expressed as means ± SEM; n = 3 per group. ^∗P < 0.05 vs the Ang II + M5Ab group; ^∗∗P < 0.05 vs the control and control + M5Ab groups. Abbreviations: Ang II, angiotensin II; MMP, matrix metalloproteinase; and L, lumen.