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. 2021 Aug 12;9:681790. doi: 10.3389/fcell.2021.681790

FIGURE 8.

FIGURE 8

OPN-N had a causal link of pyroptosis and inflammation through NF-KB mediated signaling pathways in VSMCs. (A) qPCR analyses of the mRNA expression level of NLRP3, Caspase-1, IL-1β, and IL-18 at different OPN-N concentrations. (B) Western blotting analyses of the protein levels of NF-κB in VSMCs, and quantification of NF-κB protein expression. (C,D) Western blotting analyses of the protein levels of NLRP3, Caspase-1, IL-1β, and IL-18 in VSMCs (C), and quantification of these pyroptosis-related inflammatory factors protein expression (D). β-actin was used as an internal control. (E) Immunofluorescence staining of NLRP3, Caspase-1, IL-1β, and IL-18 in mice under different treatments (left), quantification of these pyroptosis-related inflammatory factors area (right). Scar bar 50 μm. Data are expressed as means ± SEM; n = 3 per group. ∗P < 0.05 vs the Thrombin + OPN-N + SN50 group; ∗∗P < 0.05 vs the Thrombin and Thrombin + SN50 groups. Abbreviations: IL, interleukin; NF, nuclear factor; NLRP3, NOD-, LRR-, and pyrin domain-containing protein 3; OPN-N, osteopontin N-terminal fragment.