Figure 5.

Extracellular Ca²⁺ is necessary for the PMNs’ antibacterial and pro-inflammatory EV production. (A) Bacterial survival in the presence of different types of neutrophil EVs. The amount of the applied EVs was normalized to protein content. The bacterial survival of S. aureus was quantified by optical density-based method. N=3, error bars represent mean ± S.E.M. Data were compared by using RM one-way ANOVA coupled with Sidak’s post hoc test. (B) PMNs were treated for 3 h with different PMN EV populations or with controls. IL-8 amount of the supernatant was quantified with ELISA. N=3, error bars represent mean ± S.E.M. Data were compared by using RM one-way ANOVA coupled with Sidak’s multiple comparison test. *P < 0.05 and **P < 0.01. n.s., non-significant.