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. 2021 Aug 19;2021:5520644. doi: 10.1155/2021/5520644

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Copyright © 2021 Mingzhu Yang et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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A740003 inhibited the activation of NLRP3 inflammasome and phosphorylation of IKBα and decreased the expression of P2X7R in ARPE-19 cells exposed to ox-LDL for 24 hours. ELISA results showed that A740003 pretreatment decreased the secretion of inflammatory cytokines including IL-1β (to 2.831 ± 0.1162) (a) and IL-18 (to 1.585 ± 0.2329) (b) significantly. Besides, compared to the vehicle-treated group, A740003 pretreatment significantly inhibited NLRP3 (56.73% ± 0.3908) (c) and P2X7R (51.30% ± 0.1196) (d) at mRNA levels in ARPE-19 cells. It indicated that A740003 inhibited the overexpression of P2X7R and NLRP3 which was induced by ox-LDL. Western blot showed that A740003 downregulated the protein levels of NLRP3 (64.92% ± 0.1264) (e, g), pro-Caspase-1 (68.26% ± 0.02456) (f, j), Caspase-1 (65.97% ± 0.01661) (f, k), and P2X7R (60.55% ± 0.09754) (e, h) and the phosphorylation of IKBα (79.34% ± 0.01995) (e, i) significantly compared to the vehicle-treated group. Symbols: +: with; −: without. The results were mean ± SEM. Significance of difference (^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001) was determined by using one-way ANOVA with Bonferroni correction.