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. 2021 Aug 19;2021:5520644. doi: 10.1155/2021/5520644

Figure 2.

Figure 2

A740003 suppressed the upregulation of angiogenic growth factors in ARPE-19 cells induced by ox-LDL incubation for 48 hours. The mRNA levels of VEGF and HIF-1α were detected by qPCR. Compared to the vehicle-treated group, A740003 pretreatment significantly suppressed the mRNAs of VEGF (38.12% ± 0.2505) (a) and HIF-1α (30.28% ± 0.2455) (b) in ARPE-19 cells. Besides, the protein levels of VEGF and HIF-1α were detected by Western blot. A740003 pretreatment also decreased the overexpression of VEGF (51.03% ± 0.01724) (c, d) and HIF-1α (66.43% ± 0.03408) (c, e) compared to vehicle-treated cells. Symbols: +: with; −: without. The results were mean ± SEM. Significance of difference (∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001) was determined by using one-way ANOVA with Bonferroni correction.