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. 2021 Aug 19;2021:5520644. doi: 10.1155/2021/5520644

Figure 4.

Figure 4

A740003 inhibited the activation of NLRP3 inflammasome and phosphorylation of IKBα and decreased the overexpression of P2X7R in C57BL/6 mouse retinas injected with ox-LDL. The qPCR showed that compared to the vehicle-treated group, intraperitoneal injection of A740003 reduced the mRNA expression of NLRP3 (29.03% ± 0.05369) (a), Caspase-1 (39.79% ± 0.3646) (b), and P2X7R (34.73% ± 0.09999) (c) in C57BL/6 mouse retina obviously. Moreover, Western blot showed that protein levels of NLRP3 (55.03% ± 0.06134) (d, f), pro-Caspase-1 (47.16% ± 0.06827) (e, i), Caspase-1 (78.19% ± 0.0134) (e, j), IL-1β (57.09% ± 0.03169) (e, k), and P2X7R (73.04% ± 0.006164) (e, h) in C57BL/6 mouse retina were inhibited by A740003 significantly. A740003 treatment also decreased the phosphorylation of IKBα (72.25% ± 0.04076) (d, g) in C57BL/6 mouse retina. Symbols: +: with; −: without. The results were mean ± SEM. Significance of difference (∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001) was determined by using one-way ANOVA with Bonferroni correction (n = 4-6).