FIG. 1.

HA-RhoB-GG expression. (A) Western blot analysis. Extracts from different stable Rat1 and Rat1/ras transfectants were immunoblotted with the anti-HA antibody 12CA5. A total of 40 μg of cellular protein was loaded into each lane of the gels. Expression and morphology of the lines examined in each set were highly similar. (B) Northern blot analysis. Total cytoplasmic RNA for randomly selected cell lines from each set generated was fractionated by Northern analysis and hybridized to RhoB and RhoA cDNA probes.