Fig. 4.

AhR expression in mouse ICjM neurons at P5, P7, and P14. a Diagram (left) illustrating the location of the ICjM anterior to the commissural fiber (cf). A representative low-magnification image (right) of Hoechst-stained brain tissue at P5 is shown. Scale bar = 200 μm. b Representative images of immunostained sections of the ICjM at P5, P7, and P14 show distinct AhR signals in cells expressing NeuN, a marker of mature neurons (n = 3 mice at each stage). Scale bar = 100 μm. c Representative images showing the ICjM of Ahr^+/+ and Ahr^−/− mice at P14 (n = 3 mice in each genotype). Immunostained AhR signals were not observed in Ahr^−/− mice, demonstrating the specificity of the antibody. Scale bar = 100 μm