Fig. 5.

Intracellular localization of AhR in mouse ICjM neurons at P5, P7, and P14. a Immunostained ICjM neurons double-positive for NeuN and AhR. Because the cellular boundary could not be distinctly visualized, the soma areas of each neuron were not characterized. Scale bar = 10 μm. b–d Quantitative analyses of AhR-expressing ICjM neurons. The nuclear area of each neuron gradually increased with brain development (b). The AhR immunostaining intensity per nucleus (AhR^Nuc intensity) was significantly increased during the period from P5 to P14 (c). The ratio^ICjM representing AhR^Nuc intensity normalized to the nuclear area was significantly higher at P14 than those at P5 and P7, and no significant difference was observed between P5 and P7 (d). Values are shown as the mean ± SD. Circles represent individual cell data (711, 528, and 332 cells from 3 mice at each stage, P5, P7, and P14, respectively). Asterisks (***) denote statistical significance at p < 0.001 by one-way ANOVA with the Tukey–Kramer post hoc test