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. 2021 Jun 25;156(2):95–108. doi: 10.1007/s00418-021-02002-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Two “Dogiel type I” neurons (filled arrowheads) stained for neurofilaments (NF). The left one is a spiny neuron, its axon (ax) runs to the right (i.e. anally); the right one is a stubby neuron with an axon (ax) running to the left (i.e. orally). b Corresponding demonstration of staining for neuronal nitric oxide synthase (nNOS). The spiny neuron is positive, the stubby one negative (filled and empty arrowhead, respectively). c The spiny neuron is co-reactive for vasoactive intestinal peptide (VIP), the stubby neuron is negative (filled vs. empty arrowhead). d The spiny neuron is negative for choline acetyltransferase (ChAT), the stubby neuron is positive (empty vs. filled arrowhead). (From ascending colon of a 104-year-old woman; body donated to the Institute of Anatomy) Bar = 50 µm. (Antibodies: NF: Sigma-Merck N0142; nNOS: Novus Biologicals NB120-3511; VIP: Dianova T-5030; ChAT: Merck-Millipore AB144P)