Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 8;156(2):123–132. doi: 10.1007/s00418-021-01985-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — Scleral cells express tendon-associated markers. a, b Fluorescence microscopy revealed numerous scleraxis-GFP (Scx-GFP, green) positive cells in the sclera of embryonic mice at E17. b represents a magnification of the boxed area in a, highlighting the Scx-GFP positive cells (arrows). Blue = DAPI. c, d A similar situation was found in adult mice: spindle shaped Scx-GFP positive cells (green) were present throughout the entire sclera. Immunohistochemistry with CD68 revealed absence of co-localization with the Scx-GFP signal (c), while CD68-immunoreactivity was detected in few cells within the sclera (d, arrows). Blue = DAPI Scale bars: a = 100 µm, b–d = 20 µm. qRT-PCR analysis shows the expression of mRNA of the tendon-associated markers scleraxis (e), tenomodulin (Tnmd, f) and mohawk (Mkx, g), in both tendon and sclera. To a significantly lesser extent, expression is also detectable in the optic nerve.