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. 2021 Mar 29;15(9):2748–2762. doi: 10.1038/s41396-021-00967-1

Fig. 1. Experimental design.

Fig. 1

A Soil samples (n = 219) were collected from across the continental United States. B Soil slurries were created from each soil by mixing 5 g soil with 10 mL PBS, and slowly centrifuging to remove particulates. C Eight seeds were inoculated with each soil slurry, and grown on sterilized germination paper in a growth chamber. D After 1 week, all eight seedlings from one plate were combined and destructively sampled. In panels B–D, the stars indicate soil, seed, and seedling samples were analyzed with 16S rRNA gene sequencing to determine microbial community composition. Created with BioRender.com.