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. 2021 Aug 27;12:5163. doi: 10.1038/s41467-021-25384-y

Fig. 6. Chlorpyrifos inhibits diet-induced thermogenesis and β-adrenergic signaling in brown adipose tissue.

Fig. 6

a Mature brown adipocyte treated with vehicle or chlorpyrifos (CPF) or propranolol for 7 days. Fold change of cAMP levels in response to 30 min isoproterenol stimulations, n = 5. b–e Phosphorylation of HSL and p38 MAPK (b, n = 6); Zfp516 mRNA expression (c, n = 10), pULK1555, ULK, p62, LC3B-II, pACC, ACC, pAMPKaThr172 and AMPK proteins (d, e, n = 8) in BAT of C57BL/6J mice fed a HFD with 0 (control) or 2.0 mg/kg/BW (CPF-2.0) CPF and housed at thermoneutrality (TN, 30 °C) for 14weeks. f Oxygen consumption rate of brown adipocytes treated with MK-8722 and/or CPF for 6 days, n = 12. g Graphical abstract showing CPF induced obesity, non-alcoholic fatty liver disease (NAFLD), and insulin resistance by inhibiting diet-induced thermogenesis. Significant differences between three or more mean values were determined by one-way ANOVA with the post hoc Bonferroni’s multiple comparisons test; differences between two mean values were determined by a two-tailed Student’s t-test. Data presented are mean ± SEM.