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. 2021 Aug 27;11:17312. doi: 10.1038/s41598-021-96530-1

Table 1.

Lipid mediator profiles in arthritic paws of SKG mice.

Control group Arthritis group
AA derived bioactive metabolome
Prostaglandin E2 6070.3 ± 2198.0 37,005.7 ± 4476.0**
Prostaglandin D2 9122.2 ± 2720.1 37,975.4 ± 2616.2**
Prostaglandin J2 752.4 ± 118.8 2485.0 ± 291.7**
15-deoxy-Δ12,14-Prostaglandin J2 19,951.4 ± 3978.8 24,322.9 ± 4456.1
Prostaglandin F2a 2013.8 ± 371.0 16,462.7 ± 1812.3**
8iso-Prostaglandin F2a 159.7 ± 14.8 617.7 ± 57.1**
Thromboxane B2 629.3 ± 195.5 6996.9 ± 854.7**
12-HHT 259.5 ± 33.5 1874.6 ± 165.1**
Leukotriene B4 41.0 ± 11.6 165.8 ± 29.7*
Lipoxin A4
Lipoxin B4
5,15-diHETE 8.5 ± 0.6 30.5 ± 4.1**
AA pathway markers
5-HETE 104.4 ± 18.5 464.6 ± 61.3**
12-HETE 1675.0 ± 341.8 6972.2 ± 1017.4**
15-HETE 278.1 ± 39.9 1045.7 ± 134.0**
AA 5927.3 ± 642.6 15,333.5 ± 1019.6**
EPA derived bioactive metabolome
Resolvin E1 34.5 ± 16.9
Resolvin E2 9.0 ± 2.6 8.5 ± 1.7
Resolvin E3 55.4 ± 9.9**
EPA pathway markers
5-HEPE 23.0 ± 4.1 45.8 ± 6.4*
12-HEPE 84.4 ± 13.5 369.8 ± 61.1
15-HEPE 106.0 ± 28.3 208.9 ± 41.5*
18-HEPE 17.0 ± 2.8 53.1 ± 9.3*
EPA 1260.0 ± 106.1 4528.2 ± 630.5**
DHA derived bioactive metabolome
Resolvin D1 13.7 ± 3.3 45.1 ± 9.5
Resolvin D2
Resolvin D3 6.9 ± 1.4*
Resolvin D5 9.6 ± 2.9 24.7 ± 3.7*
Protectin D1 9.3 ± 3.1 10.4 ± 1.3
Maresin1 4.7 ± 2.3 5.9 ± 1.1
Maresin2 52.9 ± 15.7 108.5 ± 13.8*
DHA pathway markers
4-HDHA 45.4 ± 7.5 154.5 ± 19.7**
7-HDHA 10.7 ± 1.5 40.4 ± 5.3**
14-HDHA 350.2 ± 83.8 1210.3 ± 221.0*
17-HDHA 153.5 ± 20.1 510.7 ± 76.7*
DHA 2918.3 ± 269.8 8526.0 ± 612.1**

SKG mice were injected with or without ZyA (2 mg/body i.p.) on day 0. Paws were collected on day 112. LM profiles in paws were determined using LC/MS/MS-based LM profiling (control group, n = 5, arthritis group, n = 8). Values were represented as mean ± SEM.

**P < 0.01, *P < 0.05 by using Mann–Whitney U test and FDR-BH correction.