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. 2021 Mar 17;15(9):2627–2642. doi: 10.1038/s41396-021-00956-4

Fig. 3. Regulation of Notch signaling by P. gingivalis.

Fig. 3

A TIGK cells were infected with P. gingivalis (Pg) and expression of Notch receptor mRNA was measured by qRT-PCR. Data are expressed relative to noninfected (NI) controls. B TIGK cells were transiently transfected with siRNA to Notch receptors or scrambled siRNA (siC) and infected with P. gingivalis (Pg). OLFM4 mRNA levels were measured by qRT-PCR. Data are expressed relative to noninfected (NI) controls. C TIGK cells were infected with P. gingivalis (Pg) and expression of Notch agonist mRNA was measured by qRT-PCR. Data are expressed relative to noninfected (NI) controls. D TIGK cells were transiently transfected with siRNA to Notch agonists or scrambled siRNA (siC) and infected with P. gingivalis (Pg). OLFM4 mRNA levels were measured by qRT-PCR. Data are expressed relative to noninfected (NI) controls. E TIGK cells were transiently transfected with siRNA to Jag1 or Notch1, or scrambled siRNA (siC) and infected with P. gingivalis (Pg). Hes5 mRNA levels were measured by qRT-PCR. Data are expressed relative to noninfected (NI) control. F TIGK cells were grown in the lower and upper chambers of transwell plates. Cells in the upper chamber were transiently transfected with siRNA to Jag1 or scrambled siRNA (siC). Cells were challenged with P. gingivalis (Pg) in upper or lower chambers as indicated. Expression of OLFM4 mRNA in cells in the lower chamber was measured by qRT-PCR and expressed relative to noninfected (NI) controls. Quantitative data are means with SEM. **p < 0.01, ***p < 0.005, ****p < 0.001 compared to NI unless indicated.