Figure 1.

EFNA4 expression is associated with poor prognosis in liver cancer

(A) TCGA and two Gene Expression Omnibus datasets (GenBank: GSE121248 and GSE107170) were downloaded for EFNA4 expression analysis. EFNA4 was elevated in hepatocellular carcinoma (HCC) tissues of TCGA dataset (n = 371) compared with adjacent tissues (n = 50). ∗∗∗p < 0.001. (B) Correlation analysis of clinical T staging and EFNA4 expression in TCGA database. (C) Kaplan-Meier analysis of overall survival and progression-free survival among 370 patients with HCC. (D and E) Representative IHC images (D) and average staining scores (E) of EFNA4 expression in 90 pairs of HCC and adjacent tissues. Scale bar, 100 μm. (F and G) EFNA4 expression in HCC cell lines and normal hepatic epithelial cell line at both the RNA and protein levels by quantitative real-time PCR (F) and western blotting (G).