Figure 2.

EFNA4 enhances the replication and proliferation of HCC cell lines in vitro and in vivo

(A) Expression of EFNA4 in EFNA4-overexpressing Hep3B and Huh7 cells, as detected by quantitative real-time PCR assays. (B and C) The representative images (B) and quantitative data (C) of the EdU assay in Hep3B and Huh7. Scale bar, 50 μm. (D and E) Representative images (D) and quantitative data (E) from the flow cytometry assays. (F and G) Representative images (G) and quantitative data (F) of the subcutaneous tumor model. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (H) Representative images of H&E staining and IHC staining of Ki67. Scale bar, 50 μm.