EFNA4 promotes EMT and migration in vitro and in vivo
(A and B) Representative images and quantitative analysis of cell migration based on wound-healing assays. Scale bar, 200 μm. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (C and D) Representative images and quantitative analysis of cell migration based on Transwell assays. Scale bar, 100 μm. (E) Analysis of EMT markers by western blotting in EFNA4 overexpression cell lysates. (F and G) Representative images and quantitative analysis of the orthotropic transplantable HCC implantation model; the ellipse represents the site of tumor implantation, and the arrow represents metastasis. (H) The liver weight/body weight ratio analysis between the empty vector group and EFNA4 overexpression group. (I) Representative images of H&E staining and IHC staining of N-cadherin and E-cadherin. Scale bar, 50 μm.