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. 2021 Jun 12;25:328–341. doi: 10.1016/j.omtn.2021.06.002

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© 2021 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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The EFNA4-EPHA2-PIK3R2/GSK3β/β-catenin axis promotes DNA replication and migration of HCC cells

(A) Venn diagram of the overlap analysis of EFNA4-related molecules in TCGA database (|R| > 0.2, p < 0.05) and the results of high-throughput sequencing. (B–D) Heatmap (B), correlation analysis (C), and STRING online analysis (D) of the results of the overlap analysis. (E) Analysis of the level of EPHA2 phosphorylation by western blotting using Hep3B cell lysates. (F) EFNA4-EPHA2-PIK3R2 interactions were analyzed by coimmunoprecipitation experiments using Hep3B cell lysates, with an antibody against FLAG-EFNA4, EPHA2, or PIK3R2; interactions were revealed by western blotting. (G) Analysis of the levels of downstream molecules by western blotting using EFNA4-knockdown and EFNA4-overexpressing cell lysates.