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. 2021 Jun 12;25:328–341. doi: 10.1016/j.omtn.2021.06.002

Figure 5.

Figure 5

PIK3R2 knockdown reverses the effect of EFNA4 on the proliferation and metastasis of HCC cells

(A) PIK3R2 knockdown in EFNA4-overexpressing Hep3B and Huh7 cells, as detected by quantitative real-time PCR assays. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (B and C) Representative images and quantitative analysis of cell migration in EFNA4-overexpressing Hep3B and Huh7 cells after knockdown of PIK3R2 based on Transwell assays. Scale bar, 100 μm. (D and E) Representative images and quantitative analysis of cell migration in EFNA4-overexpressing Hep3B and Huh7 cells after knockdown of PIK3R2 based on wound-healing assays. Scale bar, 200 μm. (F and G) Representative images and quantitative data of the EdU assay in EFNA4-overexpressing Hep3B and Huh7 cells after knockdown of PIK3R2. Scale bar, 50 μm. (H) Analysis of the expression of downstream molecules by western blotting using PIK3R2-knockdown and EFNA4-overexpressing cell lysates.