Table 2.

HTL epitopes (MHC-II peptides) of CarO protein with their antigenicity, allergenicity, toxicity and mutation scores.

No	HTL epitope	Position	Vaxijen Score	Allergenicity	Toxicity	Mutation	Final decision
1	KVLRVLVTTTALLAA	16–2	− 0.2328	No allergen
2	LRVLVTTTALLAAGA	18–4	− 0.0644	No allergen
3	MKVLRVLVTTTALLA	15–1	− 0.1848	No allergen
4	VLRVLVTTTALLAAG	17–3	− 0.2461	No allergen
5	GNGDTLSIDGKNYQQ	150–131	1.8936	No allergen			*
6	LSIDGKNYQQAVPGQ	155–141	1.1512	Allergen
7	TLSIDGKNYQQAVPG	154–140	1.1523	Allergen
8	PWAQGLYIAAGAAYL	125–111	0.5259	No allergen			*
9	WAQGLYIAAGAAYLD	126–112	0.4335	No allergen			*
10	AQGLYIAAGAAYLDN	127–113	0.4818	No allergen			*
11	GLYIAAGAAYLDNDY	129–115	0.4596	No allergen			*
12	QGLYIAAGAAYLDND	128–114	0.4293	No allergen			*
13	ANEIRNDNKYEWMPV	241–226	1.7000	No allergen			*
14	GKMSYKNDIAPYLGF	175–161	0.5176	No allergen			*
15	KMSYKNDIAPYLGFG	176–162	0.4967	No allergen			*
16	RGKMSYKNDIAPYLG	174–160	0.5852	No allergen			*
17	VHDSYAFDKNQLIPV	40–26	− 0.1666	No allergen
18	VRGKMSYKNDIAPYL	173–159	0.7463	Allergen
19	IRPWGASTNPWAQGL	102–116	− 0.1476	No allergen
20	DIAPYLGFGFAPKIS	168–182	1.2750	No allergen			*
21	TALLAAGAAMADEAV	11–25	− 0.0024	No allergen
22	NPWAQGLYIAAGAAY	110–124	0.1204	No allergen
23	PWAQGLYIAAGAAYL	111–125	0.0259	No allergen
24	VHDSYAFDKNQLIPV	26–40	− 0.1666	Allergen
25	VLRVLVTTTALLAAG	3–17	− 0.2461	Allergen
26	KVLRVLVTTTALLAA	2–16	− 0.2328	Allergen
27	IAAGAAYLDNDYDLA	118–132	− 0.0341	No allergen
28	NPWAQGLYIAAGAAY	110–124	0.1204	No allergen
29	TALLAAGAAMADEAV	11–25	− 0.0024	No allergen
30	VLVTTTALLAAGAAM	6–20	− 0.0596	No allergen
31	AAGAAYLDNDYDLAK	119–133	− 0.0505	No allergen
32	LVTTTALLAAGAAMA	7–21	0.0283	Allergen
33	YLGFGFAPKISKNWG	172–186	1.7184	No allergen			*

Eleven HTL epitopes were selected (labeled with *) for selective step to incorporation in the vaccine construct