Table 3.

B-cell epitopes of CarO protein with their antigenicity and allergenicity scores

No	Bcell epitope	Position	Vaxijen Score	Allergenicity	Final decision
1	SIDGKNYQQAVPGQEGGVRG	142–161	1.6824	No allergen	*
2	LNAEIRPWGASTNPWAQGLY	98–117	0.1589	Allergen
3	KNWGVFGEVGAYYTGNPKVE	183–202	0.0053	Allergen
4	GYNGGDISWTDDVSVNGTKY	68–87	0.9330	No allergen	*
5	APVTGNPTSAQDAVDKEANE	209–228	1.1717	No allergen	*
6	NQLIPVGARAEVGTTGYGGA	35–54	0.8647	No allergen	*
7	KNQLIPV	34–40	− 0.7431	Allergen
8	IRPWGASTNRWAQGL	81–95	0.3082	Allergen
9	NVDATRSFRVNNQDFIAGADGV	113–134	0.8684	No allergen	*
10	FAPKINKNWGV	154–164	0.7523	No allergen	*
11	SGSAVTTGDQSLEEAVNA	183–200	1.2565	No allergen	*
12	DISWSDDVKVNGST	52–65	1.2709	No allergen	*
13	AMADEAVVHDSYAFDKNQLI	19–38	0.0036	Allergen
14	GAAYLDNDYDLAKRIGNGDT	121–140	0.3189	Allergen
15	WQANPYVGLALGYNGGDISW	57–76	0.4059	Allergen
16	ACINETACTERAMANN	15–30	0.5248	No allergen	*
17	EVGAYYTGNPKVELTQ	232–247	0.5643	No allergen	*
18	RAEVGTTGYGGALLWQ	85–100	0.9620	No allergen	*
19	EAVVHDSYAFDKNQLI	65–80	− 0.1973	Allergen
20	GGDISWTDDVSVNGTK	113–228	1.3248	No allergen	*
21	DNKYEWMPVGKVGVNF	274–289	0.1697	Allergen
22	PVTGNPTSAQDAVDKE	252–267	1.1236	No allergen	*
23	TQYNLAPVTGNPTSAQ	246–261	0.8548	Allergen
24	GQEGGVRGKMSYKNDI	196–211	1.9167	Allergen

Thirteen B-cell epitopes were selected (labeled with *) for selective step to incorporation in the vaccine construct