Table 5.
Allergenicity prediction of all vaccine construct using Algpred, Vaxijen and scratch protein predictor server
| No | Vaccine construct |
Complete sequence of vaccine construct | Allergenicity (Algpred) (Threshold − 0.4) |
Vaxijen Score |
SOLpro | ANTIGENpro |
|---|---|---|---|---|---|---|
| 1 | V1 | EAAAKMAKLSTDELLDAFKEMTLLELSDFVKKFEETFEVTAAAPVAVAAAGAAPAGAAVEAAEEQSEFDVILEAAGDKKIGVIKVVREIVSGLGLKEAKDLVDGAPKPLLEKVAKEAADEAKAKLEAAGATVTVKEAAAKAKFVAAWTLKAAAGGGSSIDGKNYQQAVPGQEGGVRGGGGSDISWSDDVKVNGSTGGGSGGDISWTDDVSVNGTKGGGSPVTGNPTSAQDAVDKEHEYGAEALERAGGNGDTLSIDGKNYQQHEYGAEALERAGANEIRNDNKYEWMPVHEYGAEALERAGDIAPYLGFGFAPKISHEYGAEALERAGYLGFGFAPKISKNWGHEYGAEALERAGIAPYLGFGFHEYGAEALERAGKRIGNGDTLHEYGAEALERAGWQANPYVGLHEYGAEALERAGALLWQANPYHEYGAEALERAG AKFVAAWTLKAAAGGGS |
0.22954849 Allergen |
0.8945 | 0.954742 | 0.831728 |
| 2 | V2 | EAAAKGIINTLQKYYCRVRGGRCAVLSCLPKEEQIGKCSTRGRKCCRRKKEAAAKAKFVAAWTLKAAAGGGSSIDGKNYQQAVPGQEGGVRGGGGSDISWSDDVKVNGSTGGGSGGDISWTDDVSVNGTKGGGSPVTGNPTSAQDAVDKEHEYGAEALERAGGNGDTLSIDGKNYQQHEYGAEALERAGANEIRNDNKYEWMPVHEYGAEALERAGDIAPYLGFGFAPKISHEYGAEALERAGYLGFGFAPKISKNWGHEYGAEALERAGIAPYLGFGFHEYGAEALERAGKRIGNGDTLHEYGAEALERAGWQANPYVGLHEYGAEALERAGALLWQANPYHEYGAEALERAG AKFVAAWTLKAAAGGGS |
− 0.3428515 Allergen |
1.0459 | 0.972937 | 0.875245 |
| 3 | V3 | EAAAKMAENPNIDDLPAPLLAALGAADLALATVNDLIANLRERAEETRAETRTRVEERRARLTKFQEDLPEQFIELRDKFTTEELRKAAEGYLEAATNRYNELVERGEAALQRLRSQTAFEDASARAEGYVDQAVELTQEALGTVASQTRAVGERAAKLVGIELEAAAKAKFVAAWTLKAAAGGGSSIDGKNYQQAVPGQEGGVRGGGGSDISWSDDVKVNGSTGGGSGGDISWTDDVSVNGTKGGGSPVTGNPTSAQDAVDKEHEYGAEALERAGGNGDTLSIDGKNYQQHEYGAEALERAGANEIRNDNKYEWMPVHEYGAEALERAGDIAPYLGFGFAPKISHEYGAEALERAGYLGFGFAPKISKNWGHEYGAEALERAGIAPYLGFGFHEYGAEALERAGKRIGNGDTLHEYGAEALERAGWQANPYVGLHEYGAEALERAGALLWQANPYHEYGAEALERAGAKFVAAWTLKAAAGGGS |
− 0.40767406 No allergen |
0.9251 | 0.950830 | 0.847375 |
| 4 | V4 | EAAAKMAENSNIDDIKAPLLAALGAADLALATVNELITNLRERAEETRRSRVEESRARLTKLQEDLPEQLTELREKFTAEELRKAAEGYLEAATSELVERGEAALERLRSQQSFEEVSARAEGYVDQAVELTQEALGTVASQVEGRAAKLVGIELEAAAKAKFVAAWTLKAAAGGGSSIDGKNYQQAVPGQEGGVRGGGGSDISWSDDVKVNGSTGGGSGGDISWTDDVSVNGTKGGGSPVTGNPTSAQDAVDKEHEYGAEALERAGGNGDTLSIDGKNYQQHEYGAEALERAGANEIRNDNKYEWMPVHEYGAEALERAGDIAPYLGFGFAPKISHEYGAEALERAGYLGFGFAPKISKNWGHEYGAEALERAGIAPYLGFGFHEYGAEALERAGKRIGNGDTLHEYGAEALERAGWQANPYVGLHEYGAEALERAGALLWQANPYHEYGAEALERAG AKFVAAWTLKAAAGGGS |
− 0.2874046 Allergen |
0.9330 | 0.966516 | 0.819423 |