Figure 5.

Awake breathing rodents and another odor

Anesthetized recordings (Ai–Ci), spike statistics generally increase in evoked state (time-averaged), with Ethyl Butyrate (EB, black, same as in Figures 1D–1F, 1120 cells and 17,674 pairs) and Hexanol (HX, green) with 1033 cells and 14,873 pairs. Similar format to Figure 1 with squares representing population averages. With EB, percentage of cells/pairs with evoked increases: 82%, 81.1%, 57.75% (resp. for firing rate, Var, Cov); with HX, percentage of cells/pairs with evoked increases are respectively: 87.7%, 85.3%, 49.2%. Similar plots for awake (time-averaged) recordings (Aii–Cii) from Bolding and Franks (2018a, 2018b) with same odors, 225 cells and 3,203 simultaneously recorded cell pairs (15 trials per odor), with 0.3% v/v concentration, an intermediate value in Bolding and Franks (2018a). Percentage of cells/pairs increase with EB: 51.1%, 50.7%, 52.3% (resp. for firing rate, Var, Cov), percentage of cells/pairs increase with HX: 48.4%, 50.2%, 52.3%. Spontaneous and evoked periods are 200 ms before and after inhalation, much briefer because odor concentrations dissipate rapidly (cf. 1 s odor delivery in anesthetized) and with 20 ms time windows for more samples. Evoked increases are not as prominent on average, with less percentage of cells/pairs showing the effect. Time varying statistics in anesthetized (Di–Fi) with EB (same as in Figure 1), HX (green), and awake recordings (Dii–Fii), with firing rate (D), spike count variance (E), spike count covariance (F). In (A–F), whiskers and shaded error regions are 0.2 standard deviations across population/pairs after trial-averaging for anesthetized, and 0.05 standard deviations for awake.