Figure 3.

Application of an RF classifier constructed using all the single-cell Raman spectra as training data to the classification of three prokaryotic species (B. subtilis, T. thermophilus, and N. viennensis) in a mixed population

(A) Phase-contrast micrograph of the mixed prokaryotic population, where a rod-shaped B. subtilis cell (red circled), a long T. thermophilus cell (yellow circled), and a tiny N. viennensis cell (blue circled) can be identified. Scale bar = 5 μm.

(B) Performance breakdown. Correct classification results are shown in red boxes, and misclassification results in blue boxes. The values in the table represent the number of spectra (cells). The precision and recall rates are shown in percentage.