Machine learning-assisted single-cell Raman fingerprinting for in situ and nondestructive classification of prokaryotes

. 2021 Aug 11;24(9):102975. doi: 10.1016/j.isci.2021.102975

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains

Escherichia coli	JCM	JCM 20135
Bacillus subtilis	JCM	JCM 1465^T
Thermus thermophilus	JCM	JCM 10941^T
Thermococcus kodakarensis	JCM	JCM 12380^T
Sulfolobus acidocaldarius	JCM	JCM 8929^T
Nitrososphaera viennensis	JCM	JCM 19564^T

Chemicals, peptides, and recombinant proteins

LB broth, Lennox	Nacalai tesque	Cat# 20066-95
Bacto tryptic soy broth	Becton, Dickinson and Company	Cat# 211825
Bacto peptone	Becton, Dickinson and Company	Cat# 211677
Bacto yeast extract	Becton, Dickinson and Company	Cat# 212750

Deposited data

Raman spectra of bacterial and archaeal cells	This paper; Mendeley Data	https://doi.org/10.17632/8cd34fckgt.1

Software and algorithms

Igor Pro 8.04	WaveMetrics	https://www.wavemetrics.com/RRID: SCR_014216
Python 3.7.6	Python Software Foundation	https://www.python.org/RRID: SCR_008394
scikit-learn version 0.22.1	Pedregosa et al. (2011)	https://scikit-learn.org RRID: SCR_002577
Adobe Illustrator	Adobe	https://www.adobe.com/products/illustrator.html RRID: SCR_010279