Figure 3.

Protective role of THC on the H₂O₂ induced ROS levels in HaCaT. (A) The summary of ROS levels in HaCaT cells (B) Intracellular ROS levels of HaCaT were assessed by flow cytometry. (C) α-MSH levels were found in the supernatant in HaCaT, after the cells were pretreated without or with the selected concentrations of THC (0.5, 1, 2, and 4 μg/mL) for 12 h prior to the addition of H₂O₂. Results are expressed as mean SD (n = 3). * p < 0.05 compared to the cells treated with 600 μM H₂O₂ only.