Table 5.
Phytoestrogens from pasture legume species were identified in this study by UHPLC-QTOF-MS in positive ionization mode.
| Name | Molecular Formula | Molecular Mass | [M-H] | Basis for Identification a | RT |
|---|---|---|---|---|---|
| Isoflavones | |||||
| daidzein | C15H10O4 | 254.24 | 253.0506 | STD | 8.11 |
| formononetin | C16H12O4 | 268.26 | 267.0663 | STD | 8.87 |
| genistein | C15H10O5 | 270.24 | 268.0455 | STD | 7.75 |
| Coumestans | |||||
| coumestrol | C15H8O5 | 268.22 | 267.0299 | STD | 7.88 |
| 3′methoxycoumestrol | C16H10O6 | 298.25 | 297.0405 | AM | 10.55 |
| 4′methoxycoumestrol | C16H10O5 | 282.25 | 281.0579 | AM | 8.77 |
a Basis for identification codes: AM–match to accurate mass/molecular formula; STD–match to accurate mass and retention time of analytical standards.