Table 1.
TPC, TFC, DPPH, FRAP, and α-glucosidase inhibition, activity of extracts and fractions of A. pavarii.
| Extracts/Fraction/Standard | TPC (mg GAE/g Extract) | TFC (mg QE/g Extract) | DPPH IC50 (µg/mL) | FRAP (mmol Fe (II)/g Extract) | α-glycosidase IC50 (µg/mL) |
|---|---|---|---|---|---|
| Leaf | |||||
| EX.MeOH | 886.57 ± 0.93 Aa | 442.06 ± 0.34 Ab | 17.57 ± 0.09 Aa | 82.95 ± 0.13 Aa | 8.75 ± 0.22 Ab |
| F.Hexane | 62.54 ± 0.27 Ba | 58.21 ± 0.06 Ba | 95.82 ± 0.20 Bb | 46.76 ± 0.57 Ba | ND |
| F.Chloroform | 186.94 ± 0.45 Ca | 109.09 ± 0.09 Cb | 39.50 ± 0.01 Ca | 50.43 ± 0.08 Ca | 62.64± 0.01 Ba |
| F.EtOAc | 790.76 ± 0.45 Da | 369.52 ± 0.47 Da | 6.39 ± 0. 30 Da | 86.33 ± 0.68 Da | 4.93 ± 0.09 Ca |
| F.BuOH | 390.47 ± 0.140 Eb | 277.72 ± 0.25 Ea | 27.69 ± 1.22 Eb | 82.17 ± 0.33 Aa | 10.44 ± 0.60 Db |
| Stem Bark | |||||
| EX.MeOH | 795.55 ± 1.45 Ab | 638.93 ± 0.29 Aa | 8.67 ± 0.45 Ab | 78.45 ± 0.61 Ab | 6.78 ± 0.08 Aa |
| F.Hexane | 199.14 ± 0.28 Bb | 38.41 ± 0.25 Bb | 77.07 ± 1.29 Ba | 37.59 ± 0.08 Bb | ND |
| F.Chloroform | 322.68 ± 0.01 Cb | 204.83 ± 0.34 Ca | 49.35 ± 0.01 Ca | 45.03 ± 0.75 Cb | 68.47 ± 0.06 Bb |
| F.EtOAc | 480.21± 0.13 Db | 255.463 ± 0.28 Db | 8.35 ± 0.29 Aa | 77.20 ± 0.32 Db | 5.05 ± 0.01 Ca |
| F.BuOH | 707.61± 0.58 Ea | 213.32 ±0.20 Eb | 8.71 ± 0.06 Aa | 79.19 ± 0.23 Ab | 5.90 ± 0.24 Ca |
| Quercetin | 8.6 ± 0.70 | 12.30 ± 1.40 | |||
| Ascorbic acid | 180.64 ± 0.01 | ||||
TPC, total phenolic content; TFC, total flavonoid content; DPPH, 2, 2-diphenyl-1-picrylhydrazyl; FRAP, ferric reducing antioxidant power; EX.MeOH, extract of methanol; F.Hexane, fraction of hexane; F.Chloroform, fraction of chloroform; F.EtOAc, fraction of ethyl acetate; F.BuOH, fraction of butanol. The experiment was conducted in triplicates and the result was calculated as mean ± SD. TPC assay, the curve absorbance of standard gallic acid versus sample concentrations is defined by equation y = 0.0036x + 0.0551(R2 = 0.9949). TFC assay, the curve absorbance of standard quercetin versus sample concentrations is defined by equation y = 0.0006x + 0.0326 (R2 = 0.9947). FRAP assay, the curve absorbance of standard ferrous sulfate versus sample concentrations is defined by equation y = 0.7708x + 0.0826 (R2 = 0.9987). The superscript uppercase letter is to compare the extracts and fractions of the same part. The superscript lowercase letter is to compare parts of the plant. ND = not determine.