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Estradiol decreases occludin immunoreactivity in colonic epithelium in female mice fed HFD. Colon tissues were collected on day 22 (15 days after start of HFD). Occludin immunolabeling in representative mid-colon sections from a Veh (left) and an E2-treated (right) mouse (A), Percent area (B) and mean intensity (C) of occludin immunoreactivity in the three subdivisions of the colon. Scale bar = 100 μm. * indicates differences between E2 and Veh mice (n = 6/group) (p < 0.05, t-test).
