Table 1.
Intracellular amastigote IC50 values of the pre-selection population and the 12 different post-selection clones against paromomycin (PMM) and amphotericin B (AmB). Results are expressed as the average IC50 (in µM) ± the standard error of the mean (SEM). The clones that were considered resistant (resistance index (RI) > 5) are indicated in bold. Those considered susceptible (RI < 2) are indicated in italics. All reported IC50 values are the result of at least two independent experiments run in duplicate.
| Strain | PMM IC50 (µM) 1 | AmB IC50 (nM) |
|---|---|---|
| Wild-type (preselection) | 45.0 ± 5.6 | 9.7 ± 0.2 |
| Clone 1 | 417.4 ± 15.1 | 20.9 ± 6.6 |
| Clone 2 | 196.8 ±11.2 | 15.4 ± 2.0 |
| Clone 3 | 213.0 ± 7.3 | 19.6 ± 4.5 |
| Clone 4 | 157.0 ± 9.6 | 22.4 ± 6.1 |
| Clone 5 | 129.7 ± 13.1 | 10.5 ± 1.0 |
| Clone 6 | 57.1 ± 6.8 | 11.8 ± 2.1 |
| Clone 7 | 154.0 ± 14.9 | 22.0 ± 5.1 |
| Clone 8 | 313.1 ± 14.3 | 15.3 ± 0.9 |
| Clone 9 | 132.5 ± 9.0 | 13.8 ± 0.8 |
| Clone 12 | 171.3 ± 5.7 | 13.7 ± 1.7 |
| Clone 13 | 310.2 ± 11.8 | 17.0 ± 3.5 |
| Clone 14 | 71.2 ± 2.8 | ND |
1 These results have already been published elsewhere [9]. ND: Not done.