Table 2.
Primers and cycle conditions used to amplify gene fragments.
| Pathogen | Target Gene | Primer | Sequence (5′–3′) | Cycle Conditions | Amplicon Size (bp) | Reference |
|---|---|---|---|---|---|---|
| Francisella sp. | 16S rRNA | Fr153F0.1 Fr1281R0.1 |
GCCCATTTGAGGGGGATACC GGACTAAGAGTACCTTTTTGAGT |
95 °C 4 min 40 cycles: 94 °C 30 s 60 °C 45 s 72 °C 60 s 72 °C 20 min |
1151 | [51] |
| Rickettsia sp. | ompA | RR 190-70 (1st PCR) RR 190-701(1st PCR) |
ATGGCGAATATTTCTCCAAAA GTTCCGTTAATGGCAGCATCT |
94 °C 1 min 35 cycles: 94 °C 30 s 50 °C 1 min 68 °C 4 min 72 °C 20 min |
590 | [52] |
| 190-FN1 (nested) 190-RN1 (nested) |
AAGCAATACAACAAGGTC TGACAGTTATTATACCTC |
540 | ||||
| Theileria sp. | ssrRNA | Pirop-F Pirop-F |
GTCTTGTAATTGGAATGATGG CCAAAGACTTTGATTTCTCTC |
94 °C 2 min 35 cycles: 94 °C 30 s 50 °C 30 s 72 °C 60 s 72 °C 7 min |
560 | [53] |