Table 2.

Effects of immersion time and frequency on the number of shoots per explant of R. officinalis.

Entry	Immersion Frequencies	Immersion Time (min)	No. of Shoots 1
1	every 12 h	1	170.33 ± 29.40
2	every 24 h	1	3.33 ± 1.15
3	every 12 h	5	22.00 ± 30.41
4	every 24 h	5	5.33 ± 3.06
5	every 12 h	10	5.67 ± 0.58
6	every 24 h	10	7.00 ± 7.94

¹ Average values ± SD.