Table 5.
Content of carnosol (CA) in the acetonic extracts of wild plant and callus samples of R. officinalis.
| Entry | Treatment | Retention Time (min) | Retention Factor | Absorbance (AU) | Carnosol Concentration (μg mL−1) 1 | Carnosol Concentration as mg g−1 DW 2 |
|---|---|---|---|---|---|---|
| 1 | 6-BAP (2.5 mgL−1) | 9.16 ± 0.001 | 2.77 | 0.400 ± 0.001 | 77.71 ± 0.13 | 0.7771 |
| 2 | 6-BAP (2.5 mgL−1) + 28.87 μg mL−1 CA | 9.13 ± 0.004 | 2.76 | 0.527 ± 0.006 | 101.56 ± 1.24 | - |
| 3 | 6-BAP (2.5 mgL−1) + 53.33 μg mL−1 CA | 9.15 ± 0.002 | 2.77 | 0.650 ± 0.001 | 126.27 ± 0.30 | - |
| 4 | 6-BAP (5.0 mgL−1) | 8.97 ± 0.023 | 2.69 | 0.387 ± 0.001 | 75.74 ± 0.33 | 0.7574 |
| 5 | 6-BAP (5.0 mgL−1) + 28.87 μg mL−1 CA | 9.01 ± 0.009 | 2.71 | 0.463 ± 0.004 | 90.61 ± 0.78 | - |
| 6 | 6-BAP (5.0 mgL−1) + 53.33 μg mL−1 CA | 9.00 ± 0.012 | 2.70 | 0.551 ± 0.001 | 107.1 ± 0.75 | - |
| 7 | Wild plant | 9.11 ± 0.004 | 2.75 | 0.050 ± 0.001 | 9.68 ± 0.21 | 0.0968 |
| 8 | CA (173.24 μg mL−1) | 9.21 ± 0.011 | 2.79 | 0.892 ± 0.001 | 173.24 ± 0.39 |
1 Average values ± SD. 2 The concentration of carnosol expressed as milligrams of carnosol per gram of plant dry weight (mg/g DW).