FIG. 3.
The ERα-ERβ heterodimer is activated by the mixed agonist-antagonist OHT. HeLa cells were cotransfected with the reporter construct E/GRE2ΔpS2Luc and ERα or ERβ expression vectors as indicated. The cells were treated with 100 nM OHT. Results are expressed as the percentage of the ERα-HE82 homodimer response for OHT-dependent activation. A schematic representation of the effect of OHT on the transcriptional activity of each heterodimer complex is displayed on the right of the graph. ERα and ERβ are represented as white and shaded models, respectively. Modified DBDs are depicted as black boxes.