Figure 5.

Effect of PI(3)P depletion on membranous organelle reorganization in the early phase of MCMV infection. (A) Representative (N = 12–15) focal-plane images of untreated, IN1-treated (3 µM), and solvent (DMSO)-treated uninfected and MCMV-infected (Δm138-MCMV, MOI 10) Balb/3T3 cells at 6 hpi. The cells were treated for 2 h starting at 4 hpi, fixed, permeabilized, and stained with mouse mAb to GM130 (IgG₁), rabbit mAb to Rab10, and mouse mAb to IE1 (IgG_2a), followed by non-cross reactive AF⁴⁸⁸-anti-mouse IgG₁, AF⁶⁸⁰-anti-mouse IgG_2a, and AF⁵⁵⁵-anti-rabbit IgG. (B) Representative images of cells subjected to the same procedure as in (A), treated with 10 µM IN1 instead of 3µM, and stained with rabbit polyclonal Ab against Evectin-2 (PLEKHB2) instead of anti-Rab10. Cell borders are indicated by fine dotted lines. Bars, 10 μm.