FIG. 1.
Messenger walk survey for the elimination of JNK1 and JNK2 mRNA levels following treatment with phosphorothioate antisense oligonucleotides targeted to JNK1 or JNK2 mRNA. (A) JNK1 mRNA steady-state levels in A549 cells following treatment with 26 different phosphorothioate antisense oligonucleotides targeted to JNK1 mRNA. JNK1ASISIS12539 gave the most consistent results for the elimination of JNK1 mRNA steady-state levels. (B) A similar experiment was performed with 13 phosphorothioate antisense oligonucleotides complementary to the indicated regions of the JNK2 mRNA. JNK2ASISIS12560 yielded the most consistent results for the elimination of JNK2 mRNA steady-state levels. All oligonucleotides are arrayed relative to their complementary sequence along the JNK transcript. The asterisks indicate the oligonucleotides having a nucleotide composition (2 to 4 bases) very similar to that of the leading compound. The screenings were repeated two times with similar results.