FIG. 5.

Activation of JNK1 and JNK2 by rhEGF and UV-C. A549 cells were stimulated with 0.1 μM rhEGF or UV-C at 100 J/m². Twenty minutes after stimulation, cell extracts were prepared and used for in-gel kinase assay with GST–c-Jun as a substrate, as described in Materials and Methods. The activation of each isoform (Activity) was calculated by normalization to the corresponding amount of JNK protein shown in the lower panel (Western Analysis).