FIG. 6.

Differential inhibition of JNK activity by JNK1AS and JNK2AS. A549 cells were transfected for 4.5 h with the same active antisense or control phosphorothioate 2′-O-methoxyethyl-modified oligonucleotides (0.2 μM) used in the experiments shown in Fig. 4B. Thirty-six hours after transfection, the cells were stimulated with 0.1 μM rhEGF or UV-C at 100 J/m² or were not stimulated. Twenty minutes after stimulation, cell extracts were prepared and used for a Jun kinase assay, as described in Materials and Methods. The activation is given relative to the activation obtained with rhEGF or UV-C in the scrambled-oligonucleotide-treated cells.