Table 4.
Collagen peptides identified in the acetone fraction of the hydrolysate produced with papain.
| No | Sequence | Chain Length | Mass (Da) | Net Charge | Fragment Position | Protein Name |
|---|---|---|---|---|---|---|
| 1 | AAGDAGKPGERG | 12 | 1084.5261 | −2;3 | 581–592 | Type1 collagen alpha1 chain |
| 2 | AGPAGPAGAR | 10 | 823.43005 | −2 | 1068–1077 | Type1 collagen alpha1 chain |
| 3 | AGPPGADGQAGA | 12 | 967.43592 | −2 | 807–818 | Type1 collagen alpha1 chain |
| 4 | AGPPGADGQAGAKGEPGDS | 19 | 1637.7281 | −2 | 807–825 | Type1 collagen alpha1 chain |
| 5 | AGRPGEPGPAGPPGPTGE | 18 | 1599.7641 | −2;3 | 909–926 | Type1 collagen alpha1 chain |
| 6 | AKGEPGDSGAKGDAG | 15 | 1315.6004 | −2;3 | 818–832 | Type1 collagen alpha1 chain |
| 7 | AKGETGPAGAPG | 12 | 1011.4985 | +2 | 701–712 | Type1 collagen alpha1 chain |
| 8 | AKIQLCPPPPQVPNACDMTTTV | 22 | 2437.1804 | +2 | 806–827 | Complement factor H |
| 9 | APDPFRHY | 8 | 1001.4719 | −2 | 1202–1209 | Type1 collagen alpha1 chain |
| 10 | APGEAGREGAAG | 12 | 1041.4839 | +2 | 1001–1012 | Type1 collagen alpha1 chain |
| 11 | APGEKGESGPAGPGGPTG | 18 | 1521.7059 | −2 | 770–787 | Type1 collagen alpha1 chain |
| 12 | APGEKGESGPAGPGGPTGS | 19 | 1608.738 | −2 | 770–788 | Type1 collagen alpha1 chain |
| 13 | APGFPGGPGA | 10 | 826.39735 | +2 | 335–344 | Type1 collagen alpha1 chain |
| 14 | ARGSPGSPGNDGAKGETGPAG | 21 | 1838.8507 | −2 | 689–709 | Type1 collagen alpha1 chain |
| 15 | ASGPAGPRGPAGPAGSSGKD | 20 | 1692.818 | −2;3 | 1127–1146 | Type1 collagen alpha1 chain |
| 16 | ASGPAGPRGPAGPAGSSGKDGVSG | 24 | 1992.9613 | −2;3 | 1127–1150 | Type1 collagen alpha1 chain |
| 17 | ATEAGHSAAAWLLTAQGSGTHSPL | 24 | 2333.14 | +3 | 53–76 | Peptidoglycan recognition protein 2 |
| 18 | DEGQDDRPKVGLG | 13 | 1384.6583 | +2 | 34–46 | Fibrinogen beta chain |
| 19 | DGAKGDSGPAGPKGEPGSSGE | 21 | 1855.8184 | −2;3 | 267–287 | Type1 collagen alpha1 chain |
| 20 | DGHARGDSVSQGTGLAPGSP | 20 | 1864.8664 | +3 | 270–289 | Fibrinogen alpha chain |
| 21 | DKGRLQSELKTMQD | 14 | 1647.825 | +3 | 279–292 | Cytokeratin-4 |
| 22 | DSALQLQDFYQEVANPLMTSVAF | 23 | 2586.2312 | +3 | 446–468 | Inter-alpha-trypsin inhibitor heavy... |
| 23 | DSGGPLACEKNG | 12 | 1203.519 | +2 | 576–587 | |
| 24 | EKGESGPAGPGGPT | 14 | 1239.5731 | +2 | 773–786 | Type1 collagen alpha1 chain |
| 25 | EKGEYFAFLETYGT | 14 | 1653.7563 | +2 | 336–349 | Complement component C9 |
| 26 | EKIGCSQPPQIDHG | 14 | 1564.7304 | +2 | 866–879 | Complement factor H |
| 27 | ENGLQQLTFPLSSE | 14 | 1561.7624 | +2 | 184–197 | Alpha-2-macroglobulin |
| 28 | ERGFPGE | 7 | 790.36097 | −2 | 671–677 | Type1 collagen alpha1 chain |
| 29 | EVVSLTVTCCAE | 12 | 1366.6109 | +2 | 66–77 | Vitamin D binding protein |
| 30 | EWNASQVLANLTW | 13 | 1530.7467 | +3 | 308–320 | Alpha-2-antiplasmin |
| 31 | FMQSVTGWNMGRAL | 14 | 1596.7541 | +2 | 245–258 | Angiotensinogen |
| 32 | GAAGDAGKPGERGVA | 15 | 1311.6531 | +3 | 580–594 | Type1 collagen alpha1 chain |
| 33 | GAAGPKGGPGE | 11 | 896.43519 | +2 | 493–503 | Type1 collagen alpha1 chain |
| 34 | GADGQAGAKGEPG | 13 | 1113.5051 | +2 | 811–823 | Type1 collagen alpha1 chain |
| 35 | GAKGDAGSPGPAGPTG | 16 | 1295.6106 | +2 | 826–841 | Type1 collagen alpha1 chain |
| 36 | GARGDKGEAGEAGE | 14 | 1302.58 | −2;3 | 1084–1097 | Type1 collagen alpha1 chain |
| 37 | GDRGESGPAG | 10 | 901.38897 | +2 | 1027–1036 | Type1 collagen alpha1 chain |
| 38 | GEPGDSGAKGDAGSPGPAGPTG | 22 | 1837.8078 | −2 | 820–841 | Type1 collagen alpha1 chain |
| 39 | GEPGPGGVQ | 9 | 796.37153 | −2 | 442–450 | Type1 collagen alpha1 chain |
| 40 | GEVGPAGGRGSDGPQGA | 17 | 1467.6702 | +2 | 346–362 | Type1 collagen alpha1 chain |
| 41 | GFPGADGAAGPKG | 13 | 1100.5251 | +2 | 487–499 | Type1 collagen alpha1 chain |
| 42 | GFPGPKGAAGDAGKP | 15 | 1325.6728 | +2 | 574–588 | Type1 collagen alpha1 chain |
| 43 | GGDGAPGKDGIR | 12 | 1098.5418 | +2 | 745–756 | Type1 collagen alpha1 chain |
| 44 | GGDGAPGKDGIRGM | 14 | 1286.6037 | +3 | 745–758 | Type1 collagen alpha1 chain |
| 45 | GGPGATGPAGA | 11 | 811.38243 | +2 | 679–689 | Type1 collagen alpha1 chain |
| 46 | GHRGFTGL | 8 | 843.43514 | +2 | 1102–1109 | Type1 collagen alpha1 chain |
| 47 | GIAGQRGIVG | 10 | 926.52976 | −2 | 946–955 | Type1 collagen alpha1 chain |
| 48 | GLVGPKGDTGE | 11 | 1028.5138 | +2 | 67–77 | Adiponectin |
| 49 | GMKGCPAVMPIDHVYGTLGI | 20 | 2115.0315 | +2 | 88–107 | periostin isoform X7 |
| 50 | GPAGPAGPAG | 10 | 750.36605 | −2 | 1063–1072 | Type1 collagen alpha1 chain |
| 51 | GPAGPAGSSGK | 11 | 884.43519 | +2 | 1135–1145 | Type1 collagen alpha1 chain |
| 52 | GPAGPRGPA | 9 | 778.40859 | −2 | 1129–1137 | Type1 collagen alpha1 chain |
| 53 | GPAGPRGPAGPAG | 13 | 1060.5414 | +2 | 1129–1141 | Type1 collagen alpha1 chain |
| 54 | GPMGPRGPPGPA | 12 | 1089.5389 | −2 | 175–186 | Type1 collagen alpha1 chain |
| 55 | GPMGPRGPPGPAG | 13 | 1146.5604 | −2 | 175–187 | Type1 collagen alpha1 chain |
| 56 | GPRGPPGPAG | 10 | 861.4457 | +1 | 178–187 | Type1 collagen alpha1 chain |
| 57 | GRSGRSGSFLYQ | 12 | 1313.6476 | +2 | 2406–2417 | Truncated profilaggrin |
| 58 | GRSRSFLYQVSSHE | 14 | 1651.8067 | +3 | 1436–1449 | Truncated profilaggrin |
| 59 | GSAGAQGARGDKGEAGE | 17 | 1516.6866 | −2 | 1078–1094 | Type1 collagen alpha1 chain |
| 60 | GSIQIENGYFVHYF | 14 | 1672.7886 | +3 | 251–264 | Inter-alpha-trypsin inhibitor heavy... |
| 61 | GSPGERGESGPPGPAG | 16 | 1407.6379 | +2 | 790–805 | Type1 collagen alpha1 chain |
| 62 | GSPGSPGNDGAKGETGPAG | 19 | 1611.7125 | −2 | 691–709 | Type1 collagen alpha1 chain |
| 63 | GVCISSLSCSRVGS | 14 | 1467.681 | +2 | 46–59 | |
| 64 | HRGFSGL | 7 | 772.39802 | +2 | 260–266 | Type1 collagen alpha1 chain |
| 65 | HRGFTGL | 7 | 786.41367 | −2 | 1103–1109 | Type1 collagen alpha1 chain |
| 66 | IRDVWGIEGPID | 12 | 1368.7038 | +2 | 192–203 | vitronectin |
| 67 | KGDAGSPGPAGPTG | 14 | 1167.552 | +2 | 828–841 | Type1 collagen alpha1 chain |
| 68 | KNGDRGESGPAGPAGPAGPA | 20 | 1761.8394 | −2 | 1055–1074 | Type1 collagen alpha1 chain |
| 69 | KNGDRGESGPAGPAGPAGPAG | 21 | 1818.8609 | −2 | 1055–1075 | Type1 collagen alpha1 chain |
| 70 | KNGDRGESGPAGPAGPAGPAGA | 22 | 1889.898 | −2 | 1055–1076 | Type1 collagen alpha1 chain |
| 71 | KNGDRGESGPAGPAGPAGPAGAR | 23 | 2045.9991 | −3 | 1055–1077 | Type1 collagen alpha1 chain |
| 72 | KSENARLVLQI | 11 | 1269.7405 | +3 | 158–168 | Keratin, type I cuticular Ha6 |
| 73 | LDGAKGDSGPAGPK | 14 | 1268.6361 | +3 | 266–279 | Type1 collagen alpha1 chain |
| 74 | LGIANPATDF | 10 | 1017.5131 | −2 | 728–737 | Inter-alpha-trypsin inhibitor heavy... |
| 75 | LMGEVARHSVQDGK | 14 | 1525.7671 | +3 | 103–116 | Peptidoglycan recognition protein 2 |
| 76 | LPGPTG | 6 | 540.29076 | −1 | 968–973 | |
| 77 | LPGPTGEPGKQGPGGPSGE | 19 | 1717.8271 | +2 | 968–986 | Type1 collagen alpha1 chain |
| 78 | LVDTELNCTVLQMD | 14 | 1649.7641 | +2 | 245–258 | Thyroxine-binding globulin |
| 79 | MHGLISDAEERGER | 14 | 1598.7471 | +2 | 409–422 | Keratin, type II cytoskeletal 1b |
| 80 | MSAPGPMGPMGPRGPPGPAG | 20 | 1817.8375 | +3 | 168–187 | Type1 collagen alpha1 chain |
| 81 | MSAPGPMGPMGPRGPPGPAGSN | 22 | 2018.9125 | +2 | 168–189 | Type1 collagen alpha1 chain |
| 82 | NGDRGESGPAGPAGPAGPAGA | 21 | 1761.803 | −2 | 1056–1076 | Type1 collagen alpha1 chain |
| 83 | NGDRGESGPAGPAGPAGPAGAR | 22 | 1917.9041 | −2;3 | 1056–1077 | Type1 collagen alpha1 chain |
| 84 | PAGPAGQDGRAGPPGPSGARG | 21 | 1828.8929 | +3 | 548–568 | Type1 collagen alpha1 chain |
| 85 | PGPTGEPGKQGPGGPSGE | 18 | 1604.7431 | −2 | 969–986 | Type1 collagen alpha1 chain |
| 86 | PYRVYCDMKTEKG | 13 | 1645.7592 | +2 | 269–281 | Fibrinogen beta chain |
| 87 | QLEPEE | 6 | 743.33375 | +1 | 234–239 | Complement C3 |
| 88 | RGDKGEAGEAGE | 12 | 1174.5214 | −2 | 1086–1097 | Type1 collagen alpha1 chain |
| 89 | RGEGGPAGAPGF | 12 | 1071.5098 | +2 | 624–635 | Type1 collagen alpha1 chain |
| 90 | RGESGPAGPAGPAGPAGA | 18 | 1475.7117 | +2 | 1059–1076 | Type1 collagen alpha1 chain |
| 91 | RGPPGPMGPPG | 11 | 1018.5018 | +2 | 987–997 | Type1 collagen alpha1 chain |
| 92 | RGSAGAQGARGDKGEAGE | 18 | 1672.7877 | +3 | 1077–1094 | Type1 collagen alpha1 chain |
| 93 | RGSAGAQGARGDKGEAGEA | 19 | 1743.8248 | −3 | 1077–1095 | Type1 collagen alpha1 chain |
| 94 | RGSPGSPGNDGAKGETGPAG | 20 | 1767.8136 | −2 | 690–709 | Type1 collagen alpha1 chain |
| 95 | SGAPGEAGREGAAGN | 15 | 1299.5804 | −2 | 999–1013 | Type1 collagen alpha1 chain |
| 96 | SGAPGEAGREGAAGNEGAPGRD | 22 | 1981.8838 | +2 | 999–1020 | Type1 collagen alpha1 chain |
| 97 | SGPAGPRGPAGPA | 13 | 1090.552 | +2 | 1128–1140 | Type1 collagen alpha1 chain |
| 98 | SGPPGPAG | 8 | 638.30239 | +1 | 798–805 | Type1 collagen alpha1 chain |
| 99 | SRGERGFPGERGGPGATGPAG | 21 | 1968.9514 | +3 | 668–688 | Type1 collagen alpha1 chain |
| 100 | SVMADATSVPVTE | 13 | 1305.6122 | +2 | 25–37 | Protein HP-25 homolog 2 |
| 101 | VAQPSQE | 7 | 757.36063 | −2 | 1194–1200 | Type1 collagen alpha1 chain |
| 102 | VKGGDGAPGKDGIRG | 15 | 1382.7266 | −2 | 743–757 | Type1 collagen alpha1 chain |
| 103 | VKGGDGAPGKDGIRGM | 16 | 1513.7671 | +3 | 743–758 | Type1 collagen alpha1 chain |