FIG. 2.

Production of ROIs following IL-1β stimulation. Formation of ROIs was measured using a DFCH probe in unstimulated and IL-1β-stimulated cells. Panels A, B, and C show the relative fluorescence emission at 525 nm of the DFCH probe in Raji, U937, and THP-1 cells. Columns 1, background (no DFCH probe); columns 2, unstimulated cells; columns 3, stimulation with IL-1β (50 U/ml); columns 4, as in columns 3 plus NAC (10 mM); columns 5, as in columns 3 plus NAC (20 mM); columns 6, as in columns 3 plus PDTC (60 μM); columns 7, as in columns 3 plus PDTC (100 μM); columns 8, as in columns 3 plus MK886 (0.5 μM); columns 9, as in columns 3 plus MK886 (1 μM); columns 11, stimulation with IL-1β (50 U/ml); columns 12, as in columns 11 plus ETYA (35 μM); columns 13, as in columns 11 plus ETYA (65 μM); columns 10 and 14, stimulation with H₂O₂ (250 μM). Panels D and E show the relative fluorescence emission at 525 nm of the DFCH probe in MCF7 A/Z and HCT-116 cells. Columns 1, background (no DFCH probe); columns 2, unstimulated cells; columns 3, stimulation with IL-1β (50 U/ml); columns 4, stimulation with H₂O₂ (250 μM). Asterisks indicate that the values were statistically different from the reference values (Δ).