FIG. 8.

GTPases are required for NF-κB transcriptional activity in monocytic cells. MCF7 A/Z (A), THP-1 (B), or U937 (C) cells were transfected with the HIV-κB-CAT reporter plasmid alone or together with expression vectors for wild-type or dominant-negative RhoA, Rac1, and Cdc42. Cells were either untreated (−) or stimulated with IL-1β (+) for 6 h, and CAT activities were measured. Each column represents the mean of three independent experiments (± SD). Asterisks indicate that values are statistically different from the reference values (Δ).