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. 2021 Jul 21;10(8):920. doi: 10.3390/pathogens10080920

Figure 5.

Figure 5

Inhibition of COX-2, EP2, and EP4 enhances killing of ingested MAI in macrophages. BMDM were pre-treated with NS398 (10 µM), then infected with MAI (MOI: 25) for 6 h and 24 h. Cell lysates were immunoblotted with anti-COX-2 antibody, and actin as internal control, as in (A). Viable ingested MAI in BMDM was determined at 24 h, as in (B). BMDM were pre-treated with the EP2 inhibitor and EP4 inhibitor (10 µM), then infected with MAI (MOI: 25). Viable ingested MAI in BMDM was determined at 24 h, as in (C). One-way ANOVA with Tukey’s multiple comparisons test was used, and all data are presented as mean ± SEM, * p < 0.05.