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. 2021 Jul 30;13(8):2653. doi: 10.3390/nu13082653

Figure 5.

Figure 5

Effect of human serum enriched with ALE metabolites on total intracellular lipid content in HepG2 cells. HepG2 cells were preincubated with fetal calf serum (A,B), human naive serum (C,D) or ALE enriched human serum (E,F) for 24 h and stimulated with 250µM palmitate (B,D,F) or vehicle (A,C,E) for an additional 48 h. Intracellular lipid content was analyzed using an oil red coloration. After microscopical observation, the fixed dye was redissolved in 100% isopropanol, and the optical density was measured at 490 nm in order to quantify lipidic content (G,H). Palmitate increases intracellular lipid content independently of the origin of the serum (calf or human). The human serum enriched with ALE metabolites significantly limited the palmitate-induced steatosis. **** (p < 0.0001).