Figure 8.

Effect of human serum enriched with ALE metabolites on total intracellular lipid content in primary human subcutaneous pre-adipocytes: Primary human subcutaneous pre-adipocytes were differentiated for 8 days in an adipocyte differentiation medium consisting of Preadipocyte Growth Medium-2, supplemented with insulin, dexamethasone, indomethacin and isobutyl-methylxanthine. To analyze the effects of human serum enriched with metabolites, cells were either differentiated in the presence of fetal calf serum (A,B), human naive serum (C,D) or ALE enriched human serum (E,F). Intracellular lipid content was analyzed using a red oil coloration. After microscopical observation, the fixed dye was redissolved in 100% isopropanol, and optical density was measured at 490 nm in order to quantify lipidic content (G,H). Differentiation increases intracellular lipid content independently of the origin of the serum (calf or human). The human serum enriched with ALE metabolites significantly limited the intracellular lipid accumulation in differentiated cells. ** (p < 0.01); **** (p < 0.0001).