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. 2021 Jul 30;13(8):2653. doi: 10.3390/nu13082653

Figure 10.

Figure 10

Effect of human serum enriched with ALE metabolites on AP2, PPARγ and UCP1 mRNA expression levels in primary human subcutaneous pre-adipocytes: Primary human subcutaneous pre-adipocytes were differentiated for 8 days in the presence of human serum enriched with ALE metabolites. AP2, PPARγ and UCP1 mRNA expression levels were measured by RT-PCR (PowerUp SYBRgreen, Applied Biosystems). β-Actine was used as a housekeeping gene. Primers were designed as follows: AP2-F: ATC ACA TCC CCA TTC ACA CT; AP2-R: ACT TGT CTC CAG TGA AAA CTT TG; PPARγ -F: GGA TTC AGC TGG TCG ATA TCA C; PPARγ -R: GTT TCA GAA ATG CCT TGC AGT; UCP1-F: GTC TGC CTT GAC TTT GAC AGT; UCP1-R: AGG ACC AAC GGC TTT CTT C; ACTB-F: ATT GGC AAT GAG CGG TTC; and ACTB-R: GGA TGC CAC AGG ACT CCA. Both AP2 and PPARγ mRNA expression were significantly reduced by the presence of ALE metabolites in differentiated cells. Based on UCP1 mRNA expression, the reduced white adipocyte differentiation did not involve a switch toward brown adipocyte differentiation. **** (p < 0.0001); ns: no significant difference.