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. 2021 Jul 21;10(8):919. doi: 10.3390/pathogens10080919

Figure 5.

Figure 5

Expression dynamics VACV transcripts. (a) Kinetics of mono-, bi-, and polycistronic transcripts. The B25.5R–B29R genomic region contains eight ORFs of which no LoRTIA transcripts were detected at 1 h p.i. A monocistronic (B25.5R) and a pentacistronic transcript (B25R–B28R) were expressed at 2–4 h p.i. interval. At later time points, the pentacistronic RNA and its longer overlapping partner ((a), hexacistronic transcript) are expressed; however, the monocistronic transcript disappeared. (b) Expression dynamics of the replication-associated RNAs. Several transcripts were detected at the two ends of the VACV genome, around the replication origin of the virus. These transcripts appeared at 4 h p.i., following the initiation of the DNA replication, which suggests that they might have a regulatory role in this process. See Table 1 for terminology.