Figure 6.

Categories of the expression curves of the vaccinia virus transcripts containing the same ORFs. Based on their dynamics, VACV genes can be grouped into five distinct clusters. The limitation of this approach is that only the abundant LoRTIA transcripts can be used for the analysis, while those lower-abundance overlapping transcripts that are not identified by the LoRTIA software are missing from the calculation. Other techniques (e.g., short-read sequencing, real-time RT-PCR) are not suitable for distinguishing between the overlapping transcripts and transcript isoforms; therefore, data generated by these techniques are not comparable with our results.